cell line expressing cd22 Search Results


chinese hamster ovary cho cells  (BPS Bioscience)
91
BPS Bioscience chinese hamster ovary cho cells
Dexamethasone treatment impacts the expression of LAG-3 by CD8+ T cells. PBMCs were treated with the indicated concentrations of dexamethasone (dexa), anti-PD-1 (pembrolizumab (pem)), a-CTLA-4 (ipilimumab (ipi)) alone or in combination and stimulated with SEB for 72 h. a Representative FACS plots showing expression of LAG-3 by gated CD8+ T cells in response to dexamethasone treatment and SEB stimulation. b Cumulative data of LAG-3 expression by CD8+ T cells. Data from four independent experiments shown. Error bars represent mean ± SEM. P values obtained by ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. Unstim, unstimulated cells. c Plots showing phosphorylation of the SHP-2 tyrosine phosphatase Y542 by PD-1 + Jurkat cells stimulated with <t>CHO</t> <t>cells</t> which constitutively express PD-L + and a TCR activator. d PD-1negJurkat cells were pre-treated with the indicated steroids or anti-PD-1 (pembrolizumab) alone or in combination for 48 h. Jurkat cells were co-cultured with CHO cells for 3 h at a ratio of 10:1. Cell lysates were collected followed by measurement of Y542 phosphorylation by ELISA. e PBMCs were pre-treated with the indicated steroids or anti-PD-1 (pembrolizumab) alone or in combination for 3 h. Cell lysates were collected followed by measurement of Y542 phosphorylation by ELISA. Data from four experiments shown. Error bars represent mean ± SD. P values obtained by ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test
Chinese Hamster Ovary Cho Cells, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
chinese hamster ovary cho cells - by Bioz Stars, 2026-05
91/100 stars
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cd22 cho recombinant cell line  (BPS Bioscience)
N/A
Recombinant clonal stable CHO cell line constitutively expressing full length human CD22 protein Genbank NM 001771 Surface expression of CD22 was confirmed by flow cytometry This clonal cell line was selected for medium level expression
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human cd22 knockdown cell line  (AcceGen Biotechnology)
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Human CD22 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. AcceGen offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting
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human cd22 knockout cell line  (AcceGen Biotechnology)
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Human CD22 knockout cell line is edited by CRISPR/Cas9 technology.
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Image Search Results


Dexamethasone treatment impacts the expression of LAG-3 by CD8+ T cells. PBMCs were treated with the indicated concentrations of dexamethasone (dexa), anti-PD-1 (pembrolizumab (pem)), a-CTLA-4 (ipilimumab (ipi)) alone or in combination and stimulated with SEB for 72 h. a Representative FACS plots showing expression of LAG-3 by gated CD8+ T cells in response to dexamethasone treatment and SEB stimulation. b Cumulative data of LAG-3 expression by CD8+ T cells. Data from four independent experiments shown. Error bars represent mean ± SEM. P values obtained by ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. Unstim, unstimulated cells. c Plots showing phosphorylation of the SHP-2 tyrosine phosphatase Y542 by PD-1 + Jurkat cells stimulated with CHO cells which constitutively express PD-L + and a TCR activator. d PD-1negJurkat cells were pre-treated with the indicated steroids or anti-PD-1 (pembrolizumab) alone or in combination for 48 h. Jurkat cells were co-cultured with CHO cells for 3 h at a ratio of 10:1. Cell lysates were collected followed by measurement of Y542 phosphorylation by ELISA. e PBMCs were pre-treated with the indicated steroids or anti-PD-1 (pembrolizumab) alone or in combination for 3 h. Cell lysates were collected followed by measurement of Y542 phosphorylation by ELISA. Data from four experiments shown. Error bars represent mean ± SD. P values obtained by ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: The glucocorticoids prednisone and dexamethasone differentially modulate T cell function in response to anti-PD-1 and anti-CTLA-4 immune checkpoint blockade

doi: 10.1007/s00262-020-02555-2

Figure Lengend Snippet: Dexamethasone treatment impacts the expression of LAG-3 by CD8+ T cells. PBMCs were treated with the indicated concentrations of dexamethasone (dexa), anti-PD-1 (pembrolizumab (pem)), a-CTLA-4 (ipilimumab (ipi)) alone or in combination and stimulated with SEB for 72 h. a Representative FACS plots showing expression of LAG-3 by gated CD8+ T cells in response to dexamethasone treatment and SEB stimulation. b Cumulative data of LAG-3 expression by CD8+ T cells. Data from four independent experiments shown. Error bars represent mean ± SEM. P values obtained by ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. Unstim, unstimulated cells. c Plots showing phosphorylation of the SHP-2 tyrosine phosphatase Y542 by PD-1 + Jurkat cells stimulated with CHO cells which constitutively express PD-L + and a TCR activator. d PD-1negJurkat cells were pre-treated with the indicated steroids or anti-PD-1 (pembrolizumab) alone or in combination for 48 h. Jurkat cells were co-cultured with CHO cells for 3 h at a ratio of 10:1. Cell lysates were collected followed by measurement of Y542 phosphorylation by ELISA. e PBMCs were pre-treated with the indicated steroids or anti-PD-1 (pembrolizumab) alone or in combination for 3 h. Cell lysates were collected followed by measurement of Y542 phosphorylation by ELISA. Data from four experiments shown. Error bars represent mean ± SD. P values obtained by ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test

Article Snippet: Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT with constitutive expression of PD-1 and Chinese Hamster Ovary (CHO) cells constitutively expressing PD-L1 and an engineered TCR activator were purchased from BPS Bioscience.

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay